pS375-MOP (IHC-grade phospho-µ-Opioid Receptor Antibody)

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KO-Validated

Immunohistochemical Identification of Agonist-induced Serine375 phosphorylation of the µ-Opioid Receptor in mouse spinal cord

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Order number: 7TM0319C-IC
Content: 300 µl
Host: Rabbit

Serine375 (S375) is the primary phosphorylation site in a hierarchical phosphorylation cascade.... more

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Serine375 (S375) is the primary phosphorylation site in a hierarchical phosphorylation cascade. The pS375-MOP antibody detects phosphorylation in response to high- and low-efficacy agonists but not after PKC activation. S375 phosphorylation is a key regulator of MOP desensitization, β-arrestin recruitment and internalization. The pS375-MOP antibody can detect phosphorylated MOP in mouse brain in vivo.

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Alternative Names MOP, OPRM1, µ-Opioid Receptor, Mu Receptor IUPHAR... more

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Alternative Names	MOP, OPRM1, µ-Opioid Receptor, Mu Receptor
IUPHAR Target ID	319
UniProt ID	P35372 (human) P42866 (mouse) P33535 (rat)
Immunohistochemistry (IHC)	1:50
Species Reactivity	Human, Mouse, Rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic phosphopeptide derived from human MOP around the phosphorylation site of Ser375
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Immunohistochemical Identification of Agonist-induced Serine375 phosphorylation of... more

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Figure 1. Immunohistochemical Identification of Agonist-induced Serine375 phosphorylation of the µ-Opioid Receptor in mouse spinal cord. Mice were either injected subcutaneously with saline or fentanyl citrate (0,3 mg/kg bodyweight) for 15 min. Animals were then transcardially perfused, fixed and stained in presence of phosphatase and protease inhibitors. Shown are confocal images of coronal sections of the spinal cord stained with the anti-pS375-MOP antibody (7TM0319C-IC).

Fritzwanker S, Nagel F, Kliewer A, Stammer V, Schulz S. In situ visualization of opioid and... more

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Fritzwanker S, Nagel F, Kliewer A, Stammer V, Schulz S. In situ visualization of opioid and cannabinoid drug effects using phosphosite-specific GPCR antibodies. Commun Biol. 2023 Apr 15;6(1):419. doi: 10.1038/s42003-023-04786-2. PMID: 37061609; PMCID: PMC10105690.

Radoux-Mergault A, Oberhauser L, Aureli S, Gervasio FL, Stoeber M.Subcellular location defines GPCR signal transduction. Sci Adv. 2023 Apr 21;9(16):eadf6059. doi: 10.1126/sciadv.adf6059. Epub 2023 Apr 19. PMID: 37075112; PMCID: PMC10115417.