pS425-CB1 (IHC-grade phospho-Cannabinoid Receptor 1 Antibody)

Figure 1. Immunohistochemical Identification of Agonist-induced Serine425 phosphorylation of the Cannabinoid Receptor 1 in mouse brain. Mice were either injected intraperitoneally with vehicle or specific CB1 agonist CP-55940 (0,75 mg/kg bodyweight) for 30 min. Animals were then transcardially perfused, fixed  and stained in presence of phosphatase and protease inhibitors. Shown are confocal images of coronal sections stained with the anti-pS425-CB1 antibody (7TM0056A-IC).

Figure 2. Immunohistochemical Identification of Agonist-induced Serine425 phosphorylation of the Cannabinoid Receptor 1 in mouse hippocampus. Mice were either injected intraperitoneally with vehicle or specific CB1 agonist CP-55940 (0,75 mg/kg bodyweight) for 30 min. Animals were then transcardially perfused, fixed  and stained in presence of phosphatase and protease inhibitors. Shown are confocal images of sections of hippocampal formation stained with the anti-pS425-CB1 antibody (7TM0056A-IC).

Fritzwanker S, Nagel F, Kliewer A, Stammer V, Schulz S. In situ visualization of opioid and cannabinoid drug effects using phosphosite-specific GPCR antibodies. Commun Biol. 2023 Apr 15;6(1):419. doi: 10.1038/s42003-023-04786-2. PMID: 37061609; PMCID: PMC10105690.