NK2 (IHC-grade), Tachykinin Receptor 2 Antibody

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Preview: Immunohistochemical identification of Tachykinin Receptor 2 in duodenum

Preview: Immunohistochemical identification of Tachykinin Receptor 2 in renal cell carcinoma

Preview: Validation of the Tachykinin Receptor 2 in transfected HEK293 cells

Immunohistochemical identification of Tachykinin Receptor 2 in duodenum

Immunohistochemical identification of Tachykinin Receptor 2 in renal cell carcinoma

Validation of the Tachykinin Receptor 2 in transfected HEK293 cells

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Order number: 7TM0361N-IC
Content: 100 µl
Host: Rabbit

The NK2 antibody is directed against the distal end of the carboxyl-terminal tail of human... more

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The NK2 antibody is directed against the distal end of the carboxyl-terminal tail of human Tachykinin Receptor 2. It detects selectively the NK2 Tachykinin Receptor. It can be used to detect total NK2 receptors in Western blots independent of phosphorylation. The NK2 antibody can also be used to isolate and enrich NK2 receptors from brain lysates. It also detects NK2 in cultured cells and tissue sections by immunohistochemistry.

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Alternative Names NK2, SKR, TACR2, Neurokinin B Receptor, Substance K... more

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Alternative Names	NK2, SKR, TACR2, Neurokinin B Receptor, Substance K Receptor
IUPHAR Target ID	361
UniProt ID	P21452
Western Blot (WB)	1:1000
Immunohistochemistry (IHC)	1:100
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic peptide corresponding to distal end of carboxy-terminal tail of human NK2.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Immunohistochemical identification of Tachykinin Receptor 2 in duodenum. Sections... more

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Figure 1. Immunohistochemical identification of Tachykinin Receptor 2 in duodenum. Sections were dewaxed, microwaved in citric acid, and incubated with anti-NK2 (Tachykinin Receptor 2) antibody (7TM0361N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin.

Figure 2. Immunohistochemical identification of Tachykinin Receptor 2 in renal cell carcinoma. Sections were dewaxed, microwaved in citric acid, and incubated with anti-NK2 (Tachykinin Receptor 2) antibody (7TM0361N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin.

Figure 3. Validation of the Tachykinin Receptor 2 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the Tachykinin Receptor 2 (NK2) were lysed and immunoblotted with the anti-NK2 antibody (7TM0361N-IC) at a dilution of 1:1000.

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