pT356/pT357-NK1 (phospho-Tachykinin Receptor 1 Antibody)

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Agonist-induced Threonine356/Threonine357 phosphorylation of NK1 Receptor

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Order number: 7TM0360C
Content: 100 µl
Host: Rabbit

Threonine356/Threonine357 (T356/T357) is a major phosphorylation site of the NK1 receptor.... more

Threonine356/Threonine357 (T356/T357) is a major phosphorylation site of the NK1 receptor. The pT356/pT357-NK1 antibody detects phosphorylation in response to high-efficacy agonists. T356/T357 phosphorylation is a key regulator of NK1 desensitization, β-arrestin recruitment and internalization.

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Alternative Names NK1, SPR, TACR1, Neurokinin Receptor 1, Substance P... more

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Alternative Names	NK1, SPR, TACR1, Neurokinin Receptor 1, Substance P Receptor
IUPHAR Target ID	360
UniProt ID	P25103
Western Blot (WB)	1:1000
Immunocytochemistry (ICC)	-
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic phosphopeptide derived from human NK1 around the phosphorylation site Thr356/Thr357.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Agonist-induced Threonine356/Threonine357 phosphorylation of NK1 Receptor. Upper... more

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Figure 1. Agonist-induced Threonine356/Threonine357 phosphorylation of NK1 Receptor. Upper panel, HEK293 cells stably expressing the NK1-Receptor (NK1) were either not exposed or exposed to 1 µM of specific NK1-Receptor agonist Substance P for 30 minutes. Cells were lysed and immunoblotted with the anti-pT356/pT357-NK1 antibody (7TM0360C) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-NK1 antibody (7TM0360N) at a dilution of 1:1000 to confirm equal loading of the gel.

Blum NK, Schaffner A, Drube J, Nagel F, Reinscheid RK, Hoffmann C, Schulz S. Rapid... more

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Blum NK, Schaffner A, Drube J, Nagel F, Reinscheid RK, Hoffmann C, Schulz S. Rapid elucidation of agonist-driven regulation of the neurokinin 1 receptor using a GPCR phosphorylation immunoassay. Eur J Pharmacol. 2024 Jun 15;973:176587. doi: 10.1016/j.ejphar.2024.176587. Epub 2024 Apr 19. PMID: 38642667.

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