TP (non-phospho), TP Thromboxane Receptor Antibody

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Preview: Validation of the TP Tromboxane Receptor in transfected HEK293 cells

Preview: Immunohistochemical identification of TP Tromboxane Receptor in pancreas

Validation of the TP Tromboxane Receptor in transfected HEK293 cells

Immunohistochemical identification of TP Tromboxane Receptor in pancreas

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Order number: 7TM0346N
Content: 100 µl
Host: Rabbit

The non-phospho-TP Receptor Antibody is directed against the distal end of the... more

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The non-phospho-TP Receptor Antibody is directed against the distal end of the carboxyl-terminal tail of human TP. It can be used to detect total TP receptors in Western blots independent of phosphorylation. The non-phospho-TP antibody can also be used to isolate and enrich TP receptors from cell and tissue lysates. It also detects TP in cultured cells and tissue sections by immunohistochemistry.

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Alternative Names TXA2-R, Prostanoid TP Receptor IUPHAR Target ID... more

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Alternative Names	TXA2-R, Prostanoid TP Receptor
IUPHAR Target ID	346
UniProt ID	P21731
Western Blot (WB)	1:1000
Immunohistochemistry (IHC)	1:100
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic peptide presents carboxyl-terminal tail of human TP.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Validation of the TP Tromboxane Receptor in transfected HEK293 cells . Native HEK293... more

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Figure 1. Validation of the TP Tromboxane Receptor in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the TP tromboxane receptor (TP) were lysed and immunoblotted with the phosphorylation-independent anti-TP antibody (7TM0346N) at a dilution of 1:1000.

Figure 2. Immunohistochemical identification of TP Tromboxane Receptor in pancreas. Sections were dewaxed, microwaved in citric acid, and incubated with anti-TP (non-phospho-TP Tromboxane Receptor) antibody (7TM0346N) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin.

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