EP3 (non-phospho), EP3 Prostanoid Receptor Antibody

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Validation of the EP3 Prostanoid Receptor in transfected HEK293 cells

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Order number: 7TM0342N
Content: 100 µl
Host: Rabbit

The non-phospho-EP3 receptor antibody is directed against the distal end of the... more

The non-phospho-EP3 receptor antibody is directed against the distal end of the carboxyl-terminal tail of human EP3. It can be used to detect total EP3 receptors in Western blots independent of phosphorylation.The EP3 antibody can also be used to isolate and enrich EP3 receptors from tissue lysates.

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Alternative Names PGE Receptor EP3 subtype, Prosaglandin E Receptor 3... more

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Alternative Names	PGE Receptor EP3 subtype, Prosaglandin E Receptor 3
IUPHAR Target ID	342
Uniprot ID	P43115
Western Blot (WB)	1:1000
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic peptide corresponding to c-terminal end of human EP3.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Validation of the EP3 Prostanoid Receptor in transfected HEK293 cells. Native... more

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Figure 1. Validation of the EP3 Prostanoid Receptor in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the EP3 prostanoid Receptor (EP3) were lysed and immunoblotted according to 7TM Western Blot Protocol with the phosphorylation-independent anti-EP3 antibody (7TM0342N) at a dilution of 1:1000.