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OX1 (IHC-grade) Orexin Receptor 1 Antibody

7TM Antibodies
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Immunohistochemical identification of Orexin Receptor 1 in pancreas
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  • Order number: 7TM0321N-IC
  • Content: 100 µl
  • Host: Rabbit
Description Specifications Figure Legends Citations Downloads 4 Evaluations 0
Description
The non-phospho OX1 receptor antibody is directed against the distal end of the... more
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The non-phospho OX1 receptor antibody is directed against the distal end of the carboxyl-terminal tail human OX1 receptor. It can be used to detect total OX1 receptors  in Western blots independent of phosphorylation. It can also be used to isolate and enrich OX1 receptors from tissue lysates. It also detects OX1 in cultured cells and tissue sections by immunohistochemistry.

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Specifications
  Alternative Names OX1R, Hypocretin Receptor 1 IUPHAR Target ID... more
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Alternative Names OX1R, Hypocretin Receptor 1
IUPHAR Target ID  321
UniProt ID  O43613
Western Blot (WB)  1:1000
Immunohistochemistry (IHC) 1:100
Species Reactivity   Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Immunogen A synthetic peptide presents the carboxyl-terminal tail of human OX1.
Form  Liquid
Purification Antigen affinity chromatography
Storage buffer Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions short-term 4°C, long-term -20°C
Figure Legends
Figure 1. Immunohistochemical identification of Orexin Receptor 1 in pancreas.  Sections... more
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Figure 1. Immunohistochemical identification of Orexin Receptor 1 in pancreas. Sections were dewaxed, microwaved in citric acid, and incubated with anti-OX1 (non-phospho-Orexin Receptor 1) antibody (7TM0321N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin.

 

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