pS338/pT342/pT345-M5 (phospho-M5 Muscarinic Acetylcholine Receptor Antibody)

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Agonist-induced Serine338/Threonine342/Threonine345 phosphorylation of the M5 Muscarinic Acetylcholine Receptor

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Order number: 7TM0017A
Content: 100 µl
Host: Rabbit

Serine338/Threonine342/Threonine345 is a major phosphorylation site of the M5 Muscarinic... more

Serine338/Threonine342/Threonine345 is a major phosphorylation site of the M5 Muscarinic Acetylcholine Receptor. The pS338/pT342/pT345-M5 antibody detects phosphorylation in response to high-efficacy agonists but not after PKC activation. S338/T342/T345 phosphorylation is a key regulator of M5 desensitization, β-arrestin recruitment and internalization.

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Alternative Names M5R, cholinergic receptor, muscarinic 5 IUPHAR... more

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Alternative Names	M5R, cholinergic receptor, muscarinic 5
IUPHAR Target ID	17
UniProt ID	P08912
Western Blot (WB)	1:1000
Immunocytochemistry (ICC)	-
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic phosphopeptide derived from human M5 around the phosphorylation site of Ser338/Thr342/Thr345.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Agonist-induced Serine338/Threonine342/Threonine345 phosphorylation of the M5... more

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Figure 1. Agonist-induced Serine338/Threonine342/Threonine345 phosphorylation of the M5 Muscarinic Acetylcholine Receptor. Upper panel, HEK293 cells stably expressing the M5 Muscarinic Acetylcholine Receptor (M5) and high level of GRK2 were either not exposed or exposed to 100 µM Carbachol for 30 minutes. Cells were lysed and immunoblotted according to 7TM Western Blot Protocol with the anti-pS338/pT342/pT345-M5 antibody (7TM0017A) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-M5 antibody (7TM0017N) to confirm equal loading of the gel.

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Be the first to decipher the M4 phosphorylation barcode and let us know.

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