mFFA3 (non-phospho-Mouse FFA3 Antibody)

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Validation of the mouse FFA3 in transfected HEK293 cells.

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Order number: 7TM0227MN
Content: 100 µl
Host: Rabbit

The non-phospho-FFA3 receptor antibody is directed against the distal part of the... more

The non-phospho-FFA3 receptor antibody is directed against the distal part of the carboxyl-terminal tail of mouse FFA3. It can be used to detect total FFA3 receptors in Western blots independent of phosphorylation. The non-phospho-FFA3 antibody can also be used to isolate and enrich mouse FFA3 receptors from cell and tissue lysates.

Available downloads:

Download 7TM Western Blot Protocol

Alternative Names FFAR3, Free Fatty Acid Receptor 3, GPCR41 IUPHAR... more

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Alternative Names	FFAR3, Free Fatty Acid Receptor 3, GPCR41
IUPHAR Target ID	227
UniProt ID	Q3UFD7
Western Blot (WB)	1:1000
Species Reactivity	Mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic peptide presents the carboxyl-terminal tail of mouse FFA3.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Validation of the mouse FFA3 in transfected HEK293 cells. Native HEK293 cells... more

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Figure 1. Validation of the mouse FFA3 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the mouse FFA3 Receptor (mFFA3) were lysed and immunoblotted with the phosphorylation-independent anti-mFFA3 antibody (7TM0227MN) at a dilution of 1:1000.

Barki N, Jenkins L, Marsango S, Dedeo D, Bolognini D, Dwomoh L, Abdelmalik AM, Nilsen M,... more

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Barki N, Jenkins L, Marsango S, Dedeo D, Bolognini D, Dwomoh L, Abdelmalik AM, Nilsen M, Stoffels M, Nagel F, Schulz S, Tobin AB, Milligan G. Phosphorylation bar-coding of free fatty acid receptor 2 is generated in a tissue-specific manner. Elife. 2023 Dec 12;12:RP91861. doi: 10.7554/eLife.91861. PMID: 38085667; PMCID: PMC10715726.

Ruhnke N, Beyer AL, Kaemmerer D, Sänger J, Schulz S, Lupp A. Expression of free fatty acid receptor 2 in normal and neoplastic tissues. Exp Mol Pathol. 2024 Jun;137:104902. doi: 10.1016/j.yexmp.2024.104902. Epub 2024 May 23. PMID: 38788249.

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