D5 (non-phospho), Dopamine Receptor 5 Antibody

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Preview: Validation of the Dopamine Receptor 5 in transfected HEK293 cells

Preview: Immunohistochemical identification of Dopamine Receptor 5 in mouse hippocampus

Preview: Immunohistochemical identification of Dopamine Receptor 5 in human cortex

Validation of the Dopamine Receptor 5 in transfected HEK293 cells

Immunohistochemical identification of Dopamine Receptor 5 in mouse hippocampus

Immunohistochemical identification of Dopamine Receptor 5 in human cortex

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Order number: 7TM0218N
Content: 100 µl
Host: Rabbit

The non-phospho-Dopamine Receptor 5 Antibody is directed against the distal end of the... more

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The non-phospho-Dopamine Receptor 5 Antibody is directed against the distal end of the carboxyl-terminal tail of human D5. It can be used to detect total D5 receptors in Western blots independent of phosphorylation. The non-phospho-D5 antibody can also be used to isolate and enrich D5 receptors from cell and tissue lysates. It also detects D5 in cultured cells and tissue sections by immunohistochemistry.

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Alternative Names D5, DRD1B, Dopamine Receptor 5, D(1B)Dopamine Receptor... more

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Alternative Names	D5, DRD1B, Dopamine Receptor 5, D(1B)Dopamine Receptor
IUPHAR Target ID	218
UniProt ID	P21918
Western Blot (WB)	1:1000
Immunohistochemistry (IHC)	1:100
Species Reactivity	Human, Mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic peptide presents carboxyl-terminal tail of human D5.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Validation of the Dopamine Receptor 5 in transfected HEK293 cells . Native HEK293... more

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Figure 1. Validation of the Dopamine Receptor 5 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the dopamine receptor 5 (D5) were lysed and immunoblotted with the phosphorylation-independent anti-D5 antibody (7TM0218N) at a dilution of 1:1000.

Figure 2. Immunohistochemical identification of Dopamine Receptor 5 in mouse hippocampus. Sections were dewaxed, microwaved in citric acid, and incubated with anti-D5 (Dopamine Receptor 5) antibody (7TM0218N) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin.

Figure 3. Immunohistochemical identification of Dopamine Receptor 5 in human cortex. Sections were dewaxed, microwaved in citric acid, and incubated with anti-D5 (Dopamine Receptor 5) antibody (7TM0218N) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin.

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