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- Order number: 7TM0054N
- Content: 100 µl
- Host: Rabbit
The non-phospho-CaS Calcium-sensing Receptor Antibody is directed against the distal end of the carboxyl-terminal tail of human CaS. It can be used to detect total CaS receptors in Western blots independent of phosphorylation. The non-phospho-CaS antibody can also be used to isolate and enrich CaS receptors from cell and tissue lysates. It also detects CaS in cultured cells and tissue sections by immunohistochemistry.
| Alternative Names | CaR, GPRC2A, extracellular calcium-sensing receptor, divalent cation-sensing receptor |
| IUPHAR Target ID | 54 |
| UniProt ID | P41180 |
| Western Blot (WB) | 1:1000 |
| Immunohistochemistry (IHC) | 1:100 |
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Immunogen | A synthetic peptide presents carboxyl-terminal tail of human CaS. |
| Form | Liquid |
| Purification | Antigen affinity chromatography |
| Storage buffer | Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide |
| Storage conditions | short-term 4°C, long-term -20°C |
Figure 1. Validation of the Calcium-sensing Receptor in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the Calcium-sensing receptor (CaS) were lysed and immunoblotted with the phosphorylation-independent anti-CaS antibody (7TM0054N) at a dilution of 1:1000.
Figure 2. Immunohistochemical identification of Calcium-sensing Receptor in kidney. Sections were dewaxed, microwaved in citric acid, and incubated with anti-CaS (Calcium-sensing receptor) antibody (7TM0054N) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin.
Figure 3. Immunohistochemical identification of Calcium-sensing Receptor in pancreas. Sections were dewaxed, microwaved in citric acid, and incubated with anti-CaS (Calcium-sensing receptor) antibody (7TM0054N) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin.
Figure 4. Immunohistochemical varification of Calcium-sensing Receptor antibody in human pancreas. Sections were dewaxed, microwaved in citric acid, and not exposed (left pannel) or exposed to peptide (right pannel) that was used for production of anti-CaS (non-phospho-Calcium-sensing Receptor) antibody (7TM0054N). Sections were then incubated with anti-CaS (non-phospho-Calcium-sensing Receptor) antibody (7TM0054N) at a dilution of 1:100 and sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin. Note, only in sections without peptide incubation CaS receptors were uniformly detected at the plasma membrane of nearly all cells.