pT311/pS312-A3 (phospho-A3 Adenosine Receptor Antibody)

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Agonist-induced Threonine311/Serine312 phosphorylation of the A3 Adenosine Receptor.

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Order number: 7TM0021A
Content: 100 µl
Host: Rabbit

Threonine311/Serine312 is a major phosphorylation site of the A3 Adenosine receptor.... more

Threonine311/Serine312 is a major phosphorylation site of the A3 Adenosine receptor. The pT311/pS312-A3 antibody detects phosphorylation in response to high-efficacy agonists but not after PKC activation. T311/S312 phosphorylation is a key regulator of A3 desensitization, β-arrestin recruitment and internalization.

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Alternative Names A3AR, ARA3, Adenosine receptor A3, TGPCR1 IUPHAR... more

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Alternative Names	A3AR, ARA3, Adenosine receptor A3, TGPCR1
IUPHAR Target ID	21
UniProt ID	P0DMS8
Western Blot (WB)	1:1000
Immunocytochemistry (ICC)	-
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic phosphopeptide derived from human A3 around the phosphorylation site of Thr311/Ser312.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Agonist-induced Threonine311/Serine312 phosphorylation of the A3 Adenosine Receptor.... more

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Figure 1. Agonist-induced Threonine311/Serine312 phosphorylation of the A3 Adenosine Receptor. Upper panel, HEK293 cells stably expressing the A3 Adenosine Receptor (A3) and high level of GRK2 were either not exposed or exposed to 100 nM NECA for 30 minutes. Cells were lysed and immunoblotted according to 7TM Western Blot Protocol with the anti-pT311/pS312-A3 antibody (7TM0021A) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-A3 antibody to confirm equal loading of the gel.

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