MRGPRX3 (non-phospho), MRGPRX3 Mas-related Receptor Antibody

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Preview: Validation of the MRGPRX3 Receptor in transfected HEK293 cells

Preview: Immunohistochemical identification of MRGPRX3 Receptor in cortex

Preview: Immunohistochemical identification of MRGPRX3 Receptor in heart

Preview: Immunohistochemical identification of MRGPRX3 Receptor in dudenum

Validation of the MRGPRX3 Receptor in transfected HEK293 cells

Immunohistochemical identification of MRGPRX3 Receptor in cortex

Immunohistochemical identification of MRGPRX3 Receptor in heart

Immunohistochemical identification of MRGPRX3 Receptor in dudenum

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Order number: 7TM0158N
Content: 100 µl
Host: Rabbit

The non-phospho-MRGPRX3 receptor antibody is directed against the distal end of the... more

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The non-phospho-MRGPRX3 receptor antibody is directed against the distal end of the carboxyl-terminal tail of human MRGPRX3. It can be used to detect total MRGPRX3 receptors in Western blots independent of phosphorylation. The MRGPRX3 antibody can also be used to isolate and enrich MRGPRX3 receptors from tissue lysates. It also detects MRGPRX3 in cultured cells and tissue sections by immunohistochemistry.

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Alternative Names MrgA, Mrgpra, Mrga10, Sensory neuron-specific G... more

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Alternative Names	MrgA, Mrgpra, Mrga10, Sensory neuron-specific G protein-coupled receptor 1
IUPHAR Target ID	158
Uniprot ID	Q96LB0
Western Blot (WB)	1:1000
Immunohistochemistry (IHC)	1:100
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic peptide corresponding to distal end of carboxy-terminal tail of human MRGRPX3.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Validation of the MRGPRX3 Receptor in transfected HEK293 cells. Native HEK293... more

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Figure 1. Validation of the MRGPRX3 Receptor in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the MRGPRX3 Receptor (MRGPRX3) were lysed and immunoblotted with the phosphorylation-independent anti-MRGPRX3 antibody (7TM0158N) at a dilution of 1:1000.

Figure 2. Immunohistochemical identification of MRGPRX3 Receptor in cortex. Sections were dewaxed, microwaved in citric acid, and incubated with anti-MRGPRX3 (MRGPRX3 Receptor) antibody (7TM0158N) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin.

Figure 3. Immunohistochemical identification of MRGPRX3 Receptor in heart. Sections were dewaxed, microwaved in citric acid, and incubated with anti-MRGPRX3 (MRGPRX3 Receptor) antibody (7TM0158N) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin.

Figure 4. Immunohistochemical identification of MRGPRX3 Receptor in duodenum. Sections were dewaxed, microwaved in citric acid, and incubated with anti-MRGPRX3 (MRGPRX3 Receptor) antibody (7TM0158N) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin.